Vaccine for drug addiction could offer hope to users1 day ago

CHICAGO (AFP) — In a search for what could be the ultimate cure for drug addiction, scientists have developed a vaccine which prevents the body from getting high.
The hope is that it can stop people from falling back into a spiral of addiction if they have a relapse.
The most promising results so far have been with cocaine, but researchers hope it could also one day be used to cure addiction to methamphetamine, heroin and even cigarettes.
"The vaccine slowly decreases the amount of cocaine that reaches the brain," said Thomas Kosten, a professor of psychiatry and neuroscience at Baylor College of Medicine in Houston, who has been working on the vaccine since 1995.
"It's a slow process, and patients do not go through any significant withdrawal symptoms."
The vaccine works by getting the body's immune system to recognize the drug as foreign and attack it in the blood stream.
It does so by injecting an altered version of the drug into the body which has been attached to a protein that the body will recognize as a threat.
"The body then says, 'This is a foreign article. I should start making antibodies to it,'" Kosten said in a telephone interview.
The cocaine molecules eventually pass through the kidneys and are excreted through the urine.
That stops the drug from reaching the brain and producing a sought-after high.
Use of the vaccine would lead to a gradual tapering of dependence, Kosten said.
"Gradually, antibody levels would rise. If you kept using (cocaine), you'd get less and less of an effect."
Of all the drugs tested, cocaine is the easiest one for which to develop a vaccine because of an enzyme in the bloodstream, cholinesterase, that helps break it down, Kosten said.
He has also begun to test vaccines for methamphetamine and heroin in animal studies, and hopes to eventually add nicotine to the list.
"That's going to be the moneymaker," he said.
The injections are designed for therapeutic -- not for preventative -- use, and are meant for those already suffering from addiction.
That, however, does not rule out other possible future uses, Kosten said.
"You could potentially inject pregnant cocaine users with the vaccine to prevent their fetuses from becoming contaminated," he explained.
Other uses could include administering the vaccine to high-risk adolescents in order to prevent them from becoming addicted early on, he said, while acknowledging that this would raise serious ethical and legal questions.
Testing for the cocaine vaccine has included a series of five injections over a period of three months, Kosten said.
The vaccine has one more large scale human study scheduled before it is ready for the federal Food and Drug Administration approval process.
A similar nicotine vaccine is also in the early stages of testing by several groups of European researchers. Kosten hopes to have the vaccine on the market in two to three years.

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Sampai hari ini ada sekitar 40'an Lab dalam persaingan untuk membuat antibody untuk Kokain supaya pecandu kokain tidak bisa merasakan efek dari kokain lagi.

Univeristas Baylor dari Texas menyatakan bahwa mereka hampir berhasil mengembangkan antibody untuk Kokain tetapi kenyataan yg ada ,sekitar 40'an lab dan Universitas juga dalam tahap pengembangan antibody kokain.


Ada dua strategi untuk pengembangan obat anti kokain.
1) mengunakan Antibody yg akan di produksi oleh tubuh kita dan akan melengket terhadap Molekul kokain. Jadi Molekul kokain tidak bisa melewati Blood Brain Barrier dan mempunyai efek di otak kita.
Kokain ini selanjutnya akan di metabolisme .

2) Pengembangan Enzyme yg memecahkan molekul Cocaine dgn cepat.
Latar belakang:
Cocaine molekul ada 2 Enantiomer. Seperti tangan kita ada tangan kiri dan kanan. Duanya hampir sama tapi berbeda. Duanya adalah cermin / refleksi satu sama lainnya.

Nah 2 Cocaine enatiomer itu di sebut (+) dan (-) . Yg (-) yg mempunyai efek di tubuh kita. Yg Pos tidak mempuyai efek apapun di tubuh kita.

Sewaktu di teliti , ternyata karena Cocaine (+) Di pecahkan Enzyme BChE dalam tempo 0.5 detik. Tetapi Cocaine (-) dalam tempo 40menit sampai 1 jam.
Cukup waktunya supaya Kokaine (-) bisa masuk ke otak kita dan mempunyai efek.


Nah jadi sekarang ada Lab yg mengembangkan enzim untuk tuubuh kita yg bisa memecahkan Molekul Cocaine sangking cepatnya, Cocaine tersebut tidak bisa mencapai otak kita dan No Effect. Ngak Sakaw.


ini Gambar Molekul Cocaine dan pemecahan oleh enzyme BChE
Yg di sebelah kiri bisa di lihat bahwa Ring dan Carbonyl group sudah di pecahkan.



ini gambar Cocaine molekul 3D . Yg merah melekuk itu yg akan di potong enzyme BChE dgn proses hydrolysis.






Riset ini sudah di lakukan sejak tahun 90'an .

Salah satu dari proffesor pembimbing saya adalah Researcher terkemuka untuk pengembangan Enzyme BChE . dia berkerja sama dgn 3 universitas dan 5 lab lainya.

Pengembangan ini sangat rumit dan mengunakan Super Computer modelling.
Karena enzyme itu terbuat dari protein, Tanpa super computer modelling of protein structures, hampir tidak mungkin pengembangan enzyme ini.
Misalnya ada 20 peptide di protein ini . Karena strukturnya 3 Dimensi, ada 2^20 kombinasi.
Jadi tidak mungkin mencoba 1 per satu.
didalam team ini ada org Physic, Org Kimia, org Farmasi, org Computer science , org Kedokteran, org spesialis laboratium protein. dll.

peran saya hampir nihil Karena sangking besarnya project ini . saya dari Komputer science dan matematika dan sedang mengejar Doktorate saya di Pharmacy.

Jadi jangan heran kalau beberapa tahun lagi ( bisa 3 bisa 10tahun ) ada obat anti cocaine

Oh sedikit membocor rahasia. Kolega universitas saya akan mengunakan Kmabing / Domba untuk memproduksi enzyme tersebut dari susu kambing / domba.

Kambing / domba tersebut akan di Bio Engineer dgn memasukan DNA yg akan menghasilkan enzyme tersebut di susunya